Additional file 6: of Expression profiling of Trypanosoma congolense genes during development in the tsetse fly vector Glossina morsitans morsitans

Table S3. Gene ontology analysis of differentially expressed gene products between C-parasites and PB-parasites. (XLSX 31 kb

Additional file 8: of Expression profiling of Trypanosoma congolense genes during development in the tsetse fly vector Glossina morsitans morsitans

Table S5. Expression analysis of T. congolense genes encoding cell surface proteins. (XLSX 90 kb

Additional file 5: of Expression profiling of Trypanosoma congolense genes during development in the tsetse fly vector Glossina morsitans morsitans

Table S2. Differentially expressed genes between C- and PB-parasites. Sheet 1. Differently Expressed genes (FC ≥ 2) between C-parasites and PB-parasites with FDR, P < 0.05. Sheet 2. Moderately differently expressed genes (FC < 2) between C-parasites and PB-parasites with FDR, P < 0.05. Sheet 3. Differently expressed genes (FC ≥ 2) between C-parasites and PB-parasites with FDR, P < 0.05 only with CLC-Genomics tool. Sheet 4. Differently expressed genes (FC ≥ 2) between C-parasites and PB-parasites with FDR, P < 0.05 only with EdgaR software. Sheet 5. Differently expressed genes (FC ≥ 2) between C-parasites and PB-parasites with FDR, P < 0.05 with proteomics data from [25]. (XLSX 1187 kb

Additional file 3: of Expression profiling of Trypanosoma congolense genes during development in the tsetse fly vector Glossina morsitans morsitans

Figure S1. Overview of T. congolense transcriptome analysis from infected G. morsitans cardia or proboscis. a Processing of RNA-seq reads from G. morsitans cardia or proboscis 28 days post-infection with T. congolense TC13. b Mapping statistics of T. congolense TC13 reads from G. morsitans cardia or proboscis to the T. congolense transcripts. c Spatial distribution of the most abundantly (99 percentile RPKM) expressed T. congolense TC13 genes between cardia or proboscis infections. Shared genes with higher expression in cardia or proboscis are indicated with ‘*’ in respective directions. (TIF 1642 kb

Additional file 2: of Expression profiling of Trypanosoma congolense genes during development in the tsetse fly vector Glossina morsitans morsitans

Text S2. Validation of the transcriptome. (DOCX 127 kb

Additional file 1: of Expression profiling of Trypanosoma congolense genes during development in the tsetse fly vector Glossina morsitans morsitans

Text S1. List of primers used in this study. (DOCX 105 kb

Additional file 7: of Expression profiling of Trypanosoma congolense genes during development in the tsetse fly vector Glossina morsitans morsitans

Table S4. Analysis of genes coding for proteins associated with differentiation. (XLSX 62 kb

Additional file 4: of Expression profiling of Trypanosoma congolense genes during development in the tsetse fly vector Glossina morsitans morsitans

Table S1. The top 99 percentile (RPKM) most highly expressed genes in cardia and proboscis. (XLSX 63 kb

Additional file 3: Table S3. of Tsetse fly (Glossina pallidipes) midgut responses to Trypanosoma brucei challenge

Canonical gene set enrichment analysis (GSEA) of significantly differentially expressed transcripts in the female G. pallidipes 24 or 48 h post-challenge with T. B. brucei. (XLSX 14 kb

Additional file 2: Table S2. of Tsetse fly (Glossina pallidipes) midgut responses to Trypanosoma brucei challenge

Validation of G. pallidipes RNA Seq data with qPCR RNA-seq expression values (log2 ratios) for ten genes plotted against qPCR values (log2 ratios). Figure S1. RNA-seq expression values (log2 ratios) for ten genes plotted against qPCR values (log2 ratios). (PDF 1742 kb